Interrelation between sulphur and conductive materials and its impact on ammonium and organic pollutants removal in electroactive wetlands

This investigation is the first of its kind to evaluate the interrelation of sulphate (SO₄^2-) with conductive materials as well as their individual and synergetic effects on the removal of ammonium and organic pollutants in electroactive wetlands, also known as constructed wetland (CW) - microbial fuel cell (MFC). The role of MFC components in CW was investigated to treat the sulphate containing wastewater under a long-term operation without any toxicity build-up in the system. A comparative study was also performed between CW-MFC and CW, where sulphate containing wastewater (S-replete) and without sulphate wastewater (S-deplete) was assessed. The S-replete showed high NH₄⁺ removal than the S-deplete, and the requesnce of removal was: CW-MFC-replete > CW-MFC-deplete > CW-replete > CW-deplete. The chemical oxygen demand (COD) removal was high in the case of CW-MFC-replete, and the sequence of removal was CW-MFC-replete > CW-MFC-deplete > CW-deplete > CW-replete. X-ray photon spectroscopic study indicates 0.84% sulphur accumulation in CW-MFC-replete and 2.49% in CW-replete, indicating high sulphur precipitation in CW without the MFC component. The high relative abundance of class Deltaproteobacteria (7.3%) in CW-MFC-replete along with increased microbial diversity (Shannon index = 3.5) rationalise the symbiosis of sulphate reducing/oxidising microbes and its impact on the treatment performance and electrochemical activity.