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Involvement of a transformylase enzyme in siderophore synthesis in Pseudomonas aeruginosa

journal contribution
posted on 2023-05-16, 20:24 authored by McMorran, BJ, Kumara, HM, Sullivan, K, Lamont, IL
Fluorescent pseudomonads produce yellow-green siderophores when grown under conditions of iron starvation. Here, the characterization of the pvdF gene, which is required for synthesis of the siderophore pyoverdine by Pseudomonas aeruginosa strain PAO1, is described. A P. aeruginosa pvdF mutant was constructed and found to be defective for production of pyoverdine, demonstrating the involvement of PvdF in pyoverdine synthesis. Transcription analysis showed that expression of pvdF was regulated by the amount of iron in the growth medium, consistent with its role in siderophore production. DNA sequencing showed that pvdF gives rise to a protein of 31 kDa that has similarity with glycinamide ribonucleotide transformylase (GART) enzymes involved in purine synthesis from a wide range of eukaryotic and prokaryotic species. Chemical analyses of extracts from wild-type and pvdF mutant bacteria indicated that the PvdF enzyme catalyses the formylation of N 5 -hydroxyornithine to give rise to N 5 -formyl-N 5 -hydroxyornithine, a component of pyoverdine. These studies enhance understanding of the enzymology of pyoverdine synthesis, and to the best of the authors' knowledge provide the first example of involvement of a GART-type enzyme in synthesis of a secondary metabolite.

History

Publication title

Microbiology

Volume

147

Pagination

1517-1524

ISSN

1350-0872

Department/School

Menzies Institute for Medical Research

Publisher

Soc General Microbiology

Place of publication

Marlborough House, Basingstoke Rd, Spencers Woods, Reading, England, Berks, Rg7 1Ag

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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