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Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae)
journal contribution
posted on 2023-05-17, 10:26 authored by Sakaguchi, S, Uchiyama, K, Ueno, S, Ujino-Ihara, T, Tsumura, Y, Lynda PriorLynda Prior, David BowmanDavid Bowman, Crisp, MD, Isagi, Y• Premise of the study: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan. • Methods and Results: mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing. Two hundred and nineteen primer pairs bordering sequence regions were designed from the obtained sequence data. In total, 52 showed polymorphism within 16 individuals representative of the species ’ entire range, with the number of alleles per locus and expected heterozygosity ranging from two to 10 and 0.06 to 0.84, respectively. • Conclusions: The EST-SSR markers developed in this study will be useful for evaluating the range-wide genetic structure of C. columellaris s.l. and detecting outlier loci under selection, as well as providing useful markers to investigate the conservation genetics and reproductive ecology of the species.
History
Publication title
American Journal of BotanyVolume
98Issue
12Pagination
e363-e368ISSN
0002-9122Department/School
School of Natural SciencesPublisher
Botanical Society of AmericaPlace of publication
United StatesRights statement
Copyright © 2011 CSIRORepository Status
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