Isolation and characterization of two novel ethanol-tolerant facultative-anaerobic thermophilic bacteria strains from waste compost
journal contribution
posted on 2023-05-16, 18:29authored byFong, JCN, Svenson, CJ, Nakasugi, K, Leong, CTC, John BowmanJohn Bowman, B Chen, Glenn, DR, Neilan, BA, Rogers, PL
In a search for potential ethanologens, waste compost was screened for ethanol-tolerant thermophilic microorganisms. Two thermophilic bacterial strains, M5EXG and M10EXG, with tolerance of 5 and 10% (v/v) ethanol, respectively, were isolated. Both isolates are facultative anaerobic, non-spore forming, non-motile, catalase-positive, oxidase-negative, Gram-negative rods that are capable of utilizing a range of carbon sources including arabinose, galactose, mannose, glucose and xylose and produce low amounts of ethanol, acetate and lactate. Growth of both isolates was observed in fully defined minimal media within the temperature range 50-80°C and pH 6.0-8.0. Phylogenetic analysis of the 16S rDNA sequences revealed that both isolates clustered with members of subgroup 5 of the genus Bacillus. G+C contents and DNA-DNA relatedness of M5EXG and M10EXG revealed that they are strains belonging to Geobacillus thermoglucosidasius. However, physiological and biochemical differences were evident when isolates M5EXG and M10EXG were compared with G. thermoglucosidasius type strain (DSM 2542T). The new thermophilic, ethanol-tolerant strains of G. thermoglucosidasius may be candidates for ethanol production at elevated temperatures.
History
Publication title
Extremophiles
Volume
10
Issue
5
Pagination
363-372
ISSN
1431-0651
Department/School
Tasmanian Institute of Agriculture (TIA)
Publisher
Springer Japan
Place of publication
Japan
Rights statement
Copyright 2006 Springer-Verlag
Repository Status
Restricted
Socio-economic Objectives
Environmentally sustainable energy activities not elsewhere classified