Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil
journal contribution
posted on 2023-05-16, 17:01 authored by Shane PowellShane Powell, Ma, WK, Siciliano, SDIn this study, we report the isolation of denitrifiers from hydrocarbon-contaminated Antarctic soils. Seventy-two isolates were obtained from soils that had received a fertilizer treatment to stimulate hydrocarbon degradation. All isolates, except one, belonged to the genus Pseudomonas. The one exception was a member of the Microbacteriaceae, which was also, coincidentally, the only isolate negative for the nirS gene. The diversity of the 16S rRNA and nosZ genes was assessed by denaturing gradient gel electrophoresis and sequencing. There was a slight correlation between the 16S rRNA and nosZ operational taxonomic units. Surprisingly, many isolates contained nosZ on plasmids and, to the best of our knowledge, this is the first report of nosZ being extra-chromosomally present in Pseudomonas spp. © 2006 Springer-Verlag.
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Publication title
Polar BiologyVolume
30Pagination
69-74ISSN
0722-4060Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Springer-VerlagPlace of publication
GermanyRepository Status
- Restricted
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