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Isotachophoresis on a chip with indirect fluorescence detection as a field deployable system for analysis of carboxylic acids

journal contribution
posted on 2023-05-17, 16:27 authored by Petr SmejkalPetr Smejkal, Michael BreadmoreMichael Breadmore, Rosanne Guijt, Foret, F, Bek, F, Miroslav MackaMiroslav Macka
ITP with indirect fluorescence detection (IFD) was introduced three decades ago. Despite this fact, the method has never become widely adopted. The main aim of this work was to utilize the ITP-IFD for the separation of carboxylic acids by using a commercially available, portable, microfluidic chip electrophoresis system. On the 16.8-mm effective length separation channel, a maximum of eight carboxylic acids could be separated, with LOD values in a range from 0.12 to 0.4 mM. The commercial chips used for all experiments have multichannel structures important for analysis of more than one sample per a chip in case of standard use. This multichannel structure was used to investigate the possibility of multiple sample loading for ITP separation. Application of ITP-IFD was investigated for analysis of benzoate in diet soft drinks and the results were in good agreement with results of a CE method.

History

Publication title

Electrophoresis

Volume

33

Issue

21

Pagination

3166-3172

ISSN

0173-0835

Department/School

School of Natural Sciences

Publisher

Wiley-V C H Verlag Gmbh

Place of publication

Po Box 10 11 61, Weinheim, Germany, D-69451

Rights statement

Copyright 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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