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Laser-mediated microlesions in mouse neocortex to investigate neuronal degeneration and regeneration
journal contribution
posted on 2023-05-18, 18:12 authored by Jackson, J, Alison CantyAlison Canty, Huang, L, De Paola, VIn vivo two-photon (2P) imaging enables neural circuitry to be repeatedly visualized in both normal conditions and following trauma. This protocol describes how laser-mediated neuronal microlesions can be created in the cerebral cortex using an ultrafast laser without causing a significant inflammatory reaction or compromising the blood-brain barrier. Furthermore, directives are provided for the acute and chronic in vivo imaging of the lesion site, as well as for post-hoc analysis of the lesion site in fixed tissue, which can be correlated with the live imaging phase.
History
Publication title
Current Protocols in NeuroscienceVolume
73Article number
2.24Number
2.24Pagination
2.24.1-2.24.17ISSN
1934-8584Department/School
Wicking Dementia Research Education CentrePublisher
John Wiley & Sons, Inc.Place of publication
United StatesRights statement
Copyright 2015 John Wiley & Sons, Inc.Repository Status
- Restricted