Latex-functionalized monolithic columns for the separation of carbohydrates by micro anion-exchange chromatography
journal contribution
posted on 2023-05-16, 20:04 authored by Emily HilderEmily Hilder, Svec, F, Frechet, JMJA novel stationary phase for micro ion chromatography has been prepared by coating a porous poly(butyl methacrylate-co-ethylene dimethacrylate-co-2- acrylamido-2-methyl-1-propanesulfonic acid) monolith with quaternary amine-functionalized latex particles via simple electrostatic binding. This stationary phase enabled the separation of saccharides in the mobile phase with a high-pH value consisting of aqueous ammonia solution in anion-exchange mode using evaporative light scattering for detection. Effects of both porous properties of the monolithic stationary phase and chromatographic conditions on the separation ability were studied. Under optimized conditions, an efficient separation of seven saccharides was achieved in less than 10 min. The stationary phase also enables the separation of saccharides obtained by the enzymatic hydrolysis of corn starch. © 2004 Elsevier B.V. All rights reserved.
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Publication title
Journal of Chromatography AVolume
1053Issue
1-2Pagination
101-106ISSN
0021-9673Department/School
School of Natural SciencesPublisher
ElsevierPlace of publication
AmsterdamRepository Status
- Restricted
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