University Of Tasmania

File(s) under permanent embargo

Linking oxidative and salinity stress tolerance in barley: can root antioxidant enzyme activity be used as a measure of stress tolerance?

journal contribution
posted on 2023-05-17, 12:28 authored by Maksimovic, JD, Zhang, J, Zeng, F, Branka Zivanovic, Svetlana ShabalaSvetlana Shabala, Meixue ZhouMeixue Zhou, Sergey ShabalaSergey Shabala
Aims A causal relationship between salinity and oxidative stress tolerance and a suitability of using root antioxidant activity as a biochemical marker for salinity tolerance in barley was investigated.
Methods Net ion fluxes were measured from the mature zone of excised roots of two barle varieties contrasting in their salinity tolerance using non-invasive MIFE technique in response to acute and prolonged salinity treatment. These changes were correlated with activity of major antioxidant enzymes; ascorbate peroxidase, catalase, and superoxide dismutase.
Results It was found that genotypic difference in salinity tolerance was largely independent of root integrity, and observed not only for short-term but also long-term NaCl exposures. Higher K+ retention ability (and, hence, salinity tolerance) positively correlated with oxidative stress tolerance. At the same time, antioxidant activities were constitutively higher in a sensitive but not tolerant variety, and no correlation was found between SOD activity and salinity tolerance index during large-scale screening.
Conclusion although salinity tolerance in barley correlates with its oxidative stress tolerance, higher antioxidant activity at one particular time does not correlate with salinity tolerance and, as such, cannot be used as a biochemical marker in barley screening programs.


Publication title

Plant and Soil










Tasmanian Institute of Agriculture (TIA)


Springer Netherlands

Place of publication

The Netherlands

Rights statement

Copyright Springer Science+Business Media B.V. 2012

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences