University Of Tasmania

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Low-pH and aluminum resistance in Arabidopsis correlates with high cytosolic magnesium content and increased magnesium uptake by plant roots

journal contribution
posted on 2023-05-17, 18:03 authored by Bose, J, Babourina, O, Sergey ShabalaSergey Shabala, Rengel, Z
Low-pH stress and Al3+ toxicity affect root growth in acid soils. It was hypothesized that the capacity of genotypes to maintain Mg2+ uptake in acidic environments may contribute to low-pH and Al resistance, but explicit evidence is lacking. In this work, an Al-resistant alr104 mutant and two Al-sensitive mutants (als5 and als3) of Arabidopsis thaliana were compared with the wild type (Col-0) for Mg2+ uptake and intracellular Mg2+ concentration under low-pH and combined low-pH/Al stresses. Magnesium accumulation in roots was measured in long-term (7 d) experiments. The Mg2+ fluxes were measured using ion-sensitive microelectrodes at the distal elongation and the mature root zones in short-term (0–60 min) experiments. Intracellular Mg2+ concentrations were measured in intact root cells at the distal elongation zone using magnesium-specific fluorescent dye and fluorescent lifetime imaging (FLIM) analysis. Under low-pH stress, Arabidopsis mutants als5 and alr104 maintained a higher Mg concentration in roots, and had greater Mg2+ influx than the wild type and the als3 mutant. Under combined low-pH/Al treatment, Al-resistant genotypes (wild type and alr104) maintained a higher Mg2+ accumulation, and had a higher Mg2+ influx and higher intracellular Mg2+ concentration than Al-sensitive genotypes (als3 and als5). Overall, these results show that increased Mg2+ uptake correlates with an enhanced capacity of Arabidopsis genotypes to cope with low-pH and combined low-pH/Al stresses.


Publication title

Plant and Cell Physiology










Tasmanian Institute of Agriculture (TIA)


Oxford University Press

Place of publication

Great Clarendon St, Oxford, England, Ox2 6Dp

Rights statement

copyright 2013 The Authors

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the agricultural, food and veterinary sciences