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Membrane-bound TNF supports secondary lymphoid organ structure but is subservient to secreted TNF in driving autoimmune inflammation
journal contributionposted on 2023-05-17, 07:45 authored by Ruuls, SR, Hoek, RM, Ngo, VN, McNeil, T, Lucian, LA, Janatpour, MJ, Heinrich KornerHeinrich Korner, Scheerens, H, Hessel, EM, Cyster, JG, McEvoy, LM, Sedgwick, JD
Mice without secreted TNF but with functional, normally regulated and expressed membrane-bound TNF (memTNFÎ”/Î” mice) were created by knocking-in the uncleavable Î”1-9,K11E TNF allele. In contrast to TNF-deficient mice (TNF-/-), memTNF supported many features of lymphoid organ structure, except generation of primary B cell follicles. Splenic chemokine expression was near normal. MemTNF-induced apoptosis was mediated through both TNF-R1 and TNF-R2. That memTNF is suboptimal for development of inflammation was revealed in experimental autoimmune encephalomyelitis. Disease severity was reduced in memTNFÎ”/Î” mice relative to wild-type mice, and the nature of spinal cord infiltrates resembled that in TNF-/- mice. We conclude that memTNF supports many processes underlying lymphoid tissue structure, but secreted TNF is needed for optimal inflammatory lesion development.
Department/SchoolMenzies Institute for Medical Research
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