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Methanogenium frigidum sp. nov., a psychrophilic, H-2-using methanogen from Ace lake Antarctica
journal contributionposted on 2023-05-16, 10:36 authored by Franzmann, PD, Liu, YT, Balkwill, Dl, Aldrich, HC, Demacario, EC, Boone, DR
Methanogenium frigidum sp. nov. was isolated from the perennially cold, anoxic hypolimnion of Ace Lake in the Vesfold Hills of Antarctica. The cells were psychrophilic, exhibiting most rapid growth at 15Â°C and no growth at temperatures above 18 to 20Â°C. The cells were irregular, nonmotile coccoids (diameter, 1.2 to 2.5 Î¼m) that occurred singly and grew by CO2 reduction by using H2 as a reductant. Formate could replace H2, but growth was slower. Acetate, methanol, and trimethylamine were not catabolized. Cells grew with acetate as the only organic compound in the culture medium, but growth was much faster in medium also supplemented with peptones and yeast extract. The cells were slightly halophilic; good growth occurred in medium supplemented with 350 to 600 mM Na+, but no growth occurred with 100 or 850 mM Na+. The PH range for growth was 6.5 to 7.9; no growth occurred at pH 6.0 or 8.5. Growth was slow (maximum specific growth rate, 0.24 day-1; doubling time, 2.9 days). This is the first report of a psychrophilic methanogen growing by CO2 reduction.
Publication titleInternational Journal of Systematic Bacteriology
Department/SchoolTasmanian Institute of Agriculture (TIA)
PublisherSociety for General Microbiology
Place of publicationUnited Kingdom