Methanotrophic communities in Australian woodland soils of varying salinity

Despite their large areas and potential importance as methane sinks, the role of methane-oxidizing bacteria (MOB) in native woodland soils is poorly understood. These environments are increasingly being altered by anthropogenic disturbances, which potentially alter ecosystem service provision. Dryland salinity is one such disturbance and is becoming increasingly prevalent in Australian soils. We used microarrays and analysis of soil physicochemical variables to investigate the methane-oxidizing communities of several Australian natural woodland soils affected to varying degrees by dryland salinity. Soils varied in terms of salinity, gravitational water content, NO₃-N, SO₄-S and Mg, all of which explained to a significant degree MOB community composition. Analysis of the relative abundance and diversity of the MOB communities also revealed significant differences between soils of different salinities. Type II and type Ib methanotrophs dominated the soils and differences in methanotroph communities existed between salinity groups. The low salinity soils possessed less diverse MOB communities, including most conspicuously, the low numbers or absence of type II Methylocystis phylotypes. The differences in MOB communities suggest niche separation of MOB across varying salinities, as has been observed in the closely related ammonia-oxidizing bacteria, and that anthropogenic disturbance, such as dryland salinity, has the potential to alter MOB community and therefore the methane uptake rates in soils in which disturbance occurs.