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Methodology aspects of colony maintain for a murine model of amyotrophic lateral sclerosis (ALS) TDP-43 proteinopathy

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posted on 2023-05-20, 22:08 authored by Alvaro-Alonso, C, Ferrer-Donato, A, Fernandez-Torres, E, Carballo-Villa, M, Carmen Fernandez-MartosCarmen Fernandez-Martos
The use of genetically engineered mouse (GEMs) models provides an unprecedented opportunity to study the genetic basis of diseases and gene function, therefore it is paramount to determine reproductive parameters that guarantee proper colony maintenance. We studied the reproductive parameters of mice hemizygous for TDP-43A315T transgene, which are viable, fertile, and express a mutant human TAR DNA binding protein (hTDP-43) cDNA harboring an amino acid substitution associated with familial amyotrophic lateral sclerosis (fALS). TDP43A315T mice were backcrossed to a C57Bl6/J pure background for four consecutive generations. The Tg offspring genotype were then confirmed by PCR assays. Our statistical analysis indicated there were no differences in the sex and number of pups per offspring when hemizygous female and male TDP43A315T mice were backcrossed to C57Bl6/J mice. Interestingly, our results showed significant differences in the number of offspring expressing the transgene when hemizygous TDP43A315T male mice were used as breeders. Therefore, our findings suggest that male TDP43A315T mice transfer the transgene with a greater genetic strengths. Such is an important breeding consideration to ensure the principle of reduction in animal experimentation considering most basic research with models focuses on males and excludes female mice.

History

Publication title

Animals

Volume

10

Issue

12

Article number

2329

Number

2329

Pagination

1-9

ISSN

2076-2615

Department/School

Wicking Dementia Research Education Centre

Publisher

MDPIAG

Place of publication

Switzerland

Rights statement

Copyright 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license http://creativecommons.org/licenses/by/4.0/).

Repository Status

  • Open

Socio-economic Objectives

Expanding knowledge in the health sciences

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