146257- methods for in vitro.pdf (1.83 MB)
Methods for in vitro CRISPR/CasRx-mediated RNA editing
journal contribution
posted on 2023-05-21, 01:55 authored by Chuang, Y-F, Wang, P-Y, Sarabajeet, SK, Lama, S, Fan-Li Lin, Guei-Sheung LiuGuei-Sheung LiuSpecific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more precise. Specifically, CasRx, a family member of the Cas13d family, has shown great therapeutic potential. Here, we describe the in vitro methods of utilizing this powerful RNA editing platform and determine the RNA editing efficiencies for CasRx with different forms of guide RNAs (also known as gRNA or sgRNA).
History
Publication title
Frontiers in Cell and Developmental BiologyPagination
1-13ISSN
2296-634XDepartment/School
Menzies Institute for Medical ResearchPublisher
Frontiers Research FoundationPlace of publication
SwitzerlandRights statement
Copyright © 2021 Chuang, Wang, Kumar, Lama, Lin and Liu. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) License. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these termsRepository Status
- Open
