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Methylation of ribosomal RNA genes in Petunia hybrida plants, callus cultures and regenerated shoots
journal contribution
posted on 2023-05-18, 11:31 authored by Anderson, S, Lewis-Smth, AC, Steven SmithSteven SmithThe extent of methylation of the 18S-25S ribosomal RNA genes of Petunia hybrida has been investigated using methylation-sensitive restriction endonucleases and Southern hybridisation. The genes in leaves, ovaries, petals, stigmas, anthers, and seed are methylated to a similar extent, but those in roots are hypomethylated. Genes in adventitious roots formed from stem cuttings are also hypomethylated. Genes in callus cultures initiated from leaf discs vary widely in the extent of methylation, but the pattern obtained is stable during culture. Callus cells containing hypomethylated genes can give rise to shoots in which the extent of methylation has reverted to that of the original leaf explant. These results demonstrate the striking plasticity of ribosomal RNA gene methylation in Petunia.
History
Publication title
Plant Cell ReportsVolume
8Issue
9Pagination
554-557ISSN
0721-7714Department/School
School of Natural SciencesPublisher
Springer-VerlagPlace of publication
175 Fifth Ave, New York, USA, Ny, 10010Rights statement
Copyright 1990 Springer-VerlagRepository Status
- Restricted