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MicroRNA-31 negatively regulates Interleukin-34 expression in vitro

journal contribution
posted on 2023-05-20, 04:09 authored by Li, M, Dong, Y, Chen, Z, Meng, L, Liu, X, Zhang, X, Wang, H, Mao, W, Zhang, J, Jiang, Z, Huang, T, Hu, J, Luo, P, Heinrich KornerHeinrich Korner, Ying, S, Li, J
Interleukin-34 (IL-34) is a recently discovered cytokine that promotes tissue macrophage maturation and differentiation. We previously found that 1α,25-Dihydroxyvitamin D3 up-regulated IL-34 expression in SH-SY5Y neural cells. However, whether microRNA regulates IL-34 expression is not completely clear. By using on-line TargetScan and MiRanda software, we found that there was only one conserved microRNA-31 (miR-31) binding site in the 3' untranslated region (3'UTR) of IL-34 mRNA. Intriguingly, using qPCR we demonstrated that miR-31 levels were negatively correlated to IL-34 mRNA levels in different cell lines. By examining the effect of miR-31 on IL-34 3' UTR reporter luciferase activity and on IL-34 mRNA and argonaute RISC catalytic component 2 (AGO2) binding, it was found that miR-31 bound directly to IL-34 3'UTR and regulated the post-transcriptional expression of IL-34 in MGC-803 cells. Moreover, a miR-31 mimic significantly reduced IL-34 expression levels while a miR-31 inhibitor up-regulated IL-34 expression in KYSE-45 and HT-29 cells. Taken together, these results show that miR-31 negatively regulates IL-34 expression by directly binding to the IL-34 3' UTR in vitro.


Publication title

Immunological Investigations










Menzies Institute for Medical Research


Marcel Dekker Inc

Place of publication

270 Madison Ave, New York, USA, Ny, 10016

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Copyright 2019 Taylor & Francis Group, LLC

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Clinical health not elsewhere classified

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