University Of Tasmania

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Miniature multiwavelength deep UV-LED-Based absorption detection system for capillary LC

journal contribution
posted on 2023-05-21, 10:26 authored by Hemida, M, Coates, LJ, Lam, S, Vipul GuptaVipul Gupta, Miroslav MackaMiroslav Macka, Wirth, H-J, Gooley, AA, Paul HaddadPaul Haddad
A new miniature deep UV absorbance detector has been developed using low-cost and high-performance LEDs, which can be operated in both scanning (230 to 300 nm) and individual wavelength (240, 255, and 275 nm) detection modes. The detector is mostly composed of off-the-shelf components, such as LEDs, trifurcated fiber optic assembly, a capillary Z-type flow cell, and photodiodes. It has been characterized for use with a standard capillary LC system and was benchmarked against a standard variable wavelength capillary LC detector. The detector shows very low levels of stray light (<0.4%), utilization of up to 99.0% of the effective path length of the flow cell, a wide dynamic range (0.5 to 200 μg/mL for sulfamethazine, carbamazepine, and flavone), and low noise levels (at 300 μAU level). The detector was applied within a miniaturized LC system.


Publication title

Analytical Chemistry








Tasmanian Institute of Agriculture (TIA)


Amer Chemical Soc

Place of publication

1155 16Th St, Nw, Washington, USA, Dc, 20036

Rights statement

Copyright 2020 American Chemical Society

Repository Status

  • Restricted

Socio-economic Objectives

Technological and organisational innovation; Expanding knowledge in the chemical sciences