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Multidimensional liquid-phase separations combining both chromatography and electrophoresis - A review
journal contribution
posted on 2023-05-20, 22:46 authored by Ranjbar, L, Foley, JP, Michael BreadmoreMichael BreadmoreDescribed as intrinsically powerful building blocks for two-dimensional separations by Giddings [Anal. Chem., 56 (1984) 1258A–1270A], the coupling of chromatography and electrophoresis has been proven to enhance the resolution of a wide array of molecules in complex biological, environmental and food samples. This review provides a comprehensive overview of multidimensional chromato-electrophoretic (LC - E) and electrophero-chromatographic (E - LC) separation systems from inception to the most recent published examples. LC separation modes include reversed phase, ion exchange, and size exclusion. Electromigration separation modes include capillary, microchip or free flow electrophoresis; micellar electrokinetic chromatography; electrochromatography; and isoelectric focusing. The advantages and disadvantages of various non-gel based off-line and on-line hyphenation technologies of LC - E and E - LC are discussed, with conditions and system characteristics also provided.
Funding
Australian Research Council
History
Publication title
Analytica Chimica ActaVolume
950Pagination
7-31ISSN
0003-2670Department/School
School of Natural SciencesPublisher
Elsevier Science BvPlace of publication
Po Box 211, Amsterdam, Netherlands, 1000 AeRights statement
Copyright 2016 Elsevier B.V.Repository Status
- Restricted