Ten pairs of near-isogenic lines (NILs) were developed for a major quantitative trait locus conferring Fusarium crown rot (FCR) resistance in barley. The locus locates on the long arm of chromosome 4H and the presence of the resistance allele reduced FCR severity by between 32.8 and 63.4 % with an average of 43.9 % across these NILs. Histological and quantitative PCR analyses all confirmed that the rates of Fusarium infection and disease development were much slower in the resistant isolines compared with those in the susceptible isolines. The contrasting phenotypes in FCR severity between the isolines for each of these NIL pairs would facilitate efforts in cloning and functional analyses of genes conferring resistance to this disease.
Funding
Grains Research & Development Corporation
Department of Economic Development, Jobs, Transport and Resources
History
Publication title
Euphytica
Volume
209
Pagination
555-563
ISSN
0014-2336
Department/School
Tasmanian Institute of Agriculture (TIA)
Publisher
Springer Netherlands
Place of publication
Netherlands
Rights statement
Copyright 2015 Springer Science+Business Media Dordrech