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Neoparamoeba perurans loses virulence during clonal culture
journal contribution
posted on 2023-05-18, 11:02 authored by Andrew Bridle, Davenport, D, Crosbie, PBB, Polinski, M, Barbara NowakBarbara NowakAmoebic Gill Disease affects farmed salmonids and is caused by Neoparamoeba perurans. Clonal cultures of this amoeba have been used for challenge experiments, however the effect of long-term culture on virulence has not been investigated. Here we show, using in vitro and in vivo methods, that a clone of N. perurans which was virulent 70 days after clonal culture lost virulence after 3 years in clonal culture. We propose that this is related either to the lack of attachment to the gills or the absence of an extracellular product, as shown by the lack of cytopathic effect on Chinook salmon embryo cells. The avirulent clonal culture of N. perurans allowed us to propose two potential virulence mechanisms/factors involved in Amoebic Gill Disease and is an invaluable tool for host-pathogen studies of Amoebic Gill Disease.
History
Publication title
International Journal for ParasitologyVolume
45Issue
9-10Pagination
575-578ISSN
0020-7519Department/School
Institute for Marine and Antarctic StudiesPublisher
Pergamon-Elsevier Science LtdPlace of publication
The Boulevard, Langford Lane, Kidlington, Oxford, England, Ox5 1GbRights statement
Copyright 2015 Australian Society for Parasitology Inc.Repository Status
- Open