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Non-aqueous capillary electrophoresis with red light emitting diode absorbance detection for the analysis of basic dyes

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posted on 2023-05-16, 18:47 authored by Fakhari, AR, Michael BreadmoreMichael Breadmore, Miroslav MackaMiroslav Macka, Paul HaddadPaul Haddad
Non-aqueous capillary electrophoresis was evaluated for the separation of five hydrophobic basic blue dyes for application in forensic dye analysis. The use of a red light emitting diode as a high intensity, low-noise light source provided sensitive detection of the blue dyes while also allowing the evaluation of solvents that absorb strongly in the UV region. Excellent peak shapes and separation selectivity were obtained in methanol, ethanol, acetonitrile and dimethylsulfoxide, however water, tetrahydrofuran, dimethylformamide and acetone were unsuitable as solvents due to poor peak shapes and a lack of sensitivity, most likely due to adsorption onto the capillary wall. Due to the known compatibility of methanol with capillary electrophoresis-mass spectrometry, this solvent was examined further with the relative acidity/basicity of the electrolyte being optimised with an artificial neural network. The optimised method was examined for the separation of ink samples from 6 fibre tip and 2 ball point blue or black pens and showed that a unique migration time for the main dye component in seven of the eight pens could be obtained. © 2006 Elsevier B.V. All rights reserved.

History

Publication title

Analytica Chimica Acta

Volume

580

Pagination

188-193

ISSN

0003-2670

Department/School

School of Natural Sciences

Publisher

Elsevier

Place of publication

Amsterdam

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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