University Of Tasmania
Browse
Troyer et al 2014.pdf (1.17 MB)
Download file

Novel gammaherpesviruses in North American domestic cats, bobcats and pumas: identification, prevalence and risk factors

Download (1.17 MB)
journal contribution
posted on 2023-05-17, 21:33 authored by Troyer, RM, Beatty, JA, Stutzman-Rodriguez, KR, Scott CarverScott Carver, Lozano, CC, Lee, JS, Lappin, MR, Riley, SPD, Serieys, LEK, Logan, KA, Sweanor, LL, Boyce, WM, Vickers, TW, McBride, R, Crooks, KR, Lewis, JS, Cunningham, MW, Rovnak, J, Quackenbush, SL, VandeWoude, S
Gammaherpesviruses (GHVs) are a diverse and rapidly expanding group of viruses associated with a variety of disease conditions in humans and animals. To identify felid GHVs, we screened domestic cat (Felis catus), bobcat (Lynx rufus) and puma (Puma concolor) blood cell DNA samples from California, Colorado and Florida using a degenerate pan-GHV PCR. Additional pan-GHV and long-distance PCRs were used to sequence a contiguous 3.4 kb region of each putative virus species including partial glycoprotein B and DNA polymerase genes. We identified three novel GHVs, each present predominantly in one felid species: Felis catus GHV 1 (FcaGHV1) in domestic cats, Lynx rufus GHV 1 (LruGHV1) in bobcats, and Puma concolor GHV 1 (PcoGHV1) in pumas. To estimate infection prevalence, we developed real-time quantitative PCR assays for each virus and screened additional DNA samples from all three species (n = 282). FcaGHV1 was detected in 16% of domestic cats across all study sites. LruGHV1 was detected in 47% of bobcats and 13% of pumas across all study sites, suggesting relatively common interspecific transmission. PcoGHV1 was detected in 6% of pumas, all from a specific region of Southern California. The risk of infection for each host varied with geographic location. Age was a positive risk factor for bobcat LruGHV1 infection, and age and being male were risk factors for domestic cat FcaGHV1 infection. Further characterization of these viruses may have significant health implications for domestic cats and may aid studies of free-ranging felid ecology.

History

Publication title

Journal of Virology

Volume

88

Issue

8

Pagination

3914-3924

ISSN

0022-538X

Department/School

School of Natural Sciences

Publisher

Amer Soc Microbiology

Place of publication

1752 N St Nw, Washington, USA, Dc, 20036-2904

Rights statement

Copyright 2014 American Society for Microbiology

Repository Status

  • Open

Socio-economic Objectives

Disease distribution and transmission (incl. surveillance and response)

Usage metrics

    University Of Tasmania

    Exports