File(s) under permanent embargo
Oral administration of glucose promotes intracellular partitioning of fatty acid toward storage in white but not in red muscle
journal contribution
posted on 2023-05-17, 13:47 authored by Fosgerau, K, Fledelius, C, Fledelius, KE, Kristensen, B, Daugaard, JR, Miguel IglesiasMiguel Iglesias, Kraegen, EW, Furler, SMLipid accumulation in non-adipose tissues is strongly associated with the metabolic syndrome, possibly due to aberrant partitioning of intracellular fatty acids between storage and oxidation. In the present study, we administered the non-metabolizable fatty acid analog [9,10-(3)H]-(R)-2-bromopalmitate, and authentic (14)C-palmitate to conscious rats, in order to directly examine the initial intracellular fate of fatty acids in a range of insulin-sensitive tissues, including white and red muscles, liver, white adipose tissue, and heart. Rats were studied after administration of an oral glucose load to examine the effect of physiological elevation of glucose and insulin. The tracer results showed that glucose administration partitioned fatty acid toward storage in white muscle (storage:uptake ratios, vehicle vs glucose; 0.64 +/- 0.02 vs 0.92 +/- 0.09, P < 0.05), and in liver (0.66 +/- 0.07 vs 0.98 +/- 0.04, P < 0.05), but not in red muscle (1.18 +/- 0.07 vs 1.36 +/- 0.11, P = not significant). These results demonstrate the physiological relevance of the so-called 'reverse' Randle cycle, but surprisingly show that it may be more important in white rather than oxidative red muscle.
History
Publication title
Journal of EndocrinologyVolume
190Pagination
651-8ISSN
0022-0795Department/School
School of Health SciencesPublisher
Soc EndocrinologyPlace of publication
22 Apex Court, Woodlands, Bradley Stoke, Bristol, England, Bs32 4JtRights statement
Copyright 2006 Society for EndocrinologyRepository Status
- Restricted