File(s) under permanent embargo
Oral vaccination of first-feeding Atlantic salmon, Salmo salar L., confers greater protection against yersiniosis than immersion vaccination
journal contribution
posted on 2023-05-18, 16:00 authored by Bikramjit GhoshBikramjit Ghosh, Nguyen, TD, Crosbie, PBB, Barbara NowakBarbara Nowak, Andrew BridleAndrew BridleYersinia ruckeri is a ubiquitous pathogen of finfish capable of causing major mortalities in farmed fish stocks. It can be transmitted vertically from parent to progeny as well as horizontally in the water column from both clinically infected fish and asymptomatic carriers, and is consequently capable of infecting fish at early stages of development. Immunisation strategies that can protect small fry are therefore critical for the effective management of fish health, as is the ability to detect covertly infected fish. In this study, first-feeding Atlantic salmon fry (< 0.5 g) were immunised either by oral administration of a microencapsulated Y. ruckeri vaccine formulation (0.38 g initial weight), or via immersion in bacterin suspension (0.26 g), with and without a booster immersion vaccination at 1 g size. Protection in groups receiving only immersion immunisation did not differ significantly from untreated controls when challenged with Y. ruckeri at approximately 5 g size, while orally immunised fish were significantly better protected than untreated controls (F = 4.38, df = 4,10, P = 0.026), with RPS varying between 29.4% (ORAL) and 51% (ORAL + DIP). A quantitative real-time PCR assay was used to successfully detect covertly infected fish among challenge survivors, indicating more than 50% of surviving fish in each group were infected with no significant differences between immunised fish and untreated controls.
History
Publication title
VaccineVolume
34Issue
5Pagination
599-608ISSN
0264-410XDepartment/School
Institute for Marine and Antarctic StudiesPublisher
Elsevier Sci LtdPlace of publication
The Boulevard, Langford Lane, Kidlington, Oxford, England, Oxon, Ox5 1GbRights statement
© 2015 Elsevier Ltd. All rights reserved.Repository Status
- Restricted