235.pdf (211.84 kB)
Download filePeak shapes in open-tubular ion-exchange capillary electrochromatography of inorganic anions
journal contribution
posted on 2023-05-16, 12:16 authored by Michael BreadmoreMichael Breadmore, Boyce, M, Miroslav MackaMiroslav Macka, Avdalovic, N, Paul HaddadPaul HaddadAn experimental study of parameters influencing peak shapes in ion-exchange open tubular (OT) capillary electrochromatography (CEC) was conducted using adsorbed quaternary aminated latex particles as the stationary phase. The combination of separation mechanisms from both capillary electrophoresis and ion-exchange chromatography results in peak broadening in OT-CEC arising from both these techniques. The sources of peak broadening that were considered included the relative electrophoretic mobilities of the eluent co-ion and analyte, and resistance to mass transfer in both the mobile and stationary phases. The parameters investigated were the mobility of the eluent co-ion, column diameter, separation temperature and secondary interactions between the analyte and the stationary phase. The electromigration dispersion was found to influence peak shapes to a minor extent, indicating that chromatographic retention was the dominant source of dispersion. Improving the resistance to mass transfer in the mobile phase by decreasing the capillary diameter improved peak shapes, with symmetrical peaks being obtained in a 25 μm I.D. column. However, an increase in temperature from 25°C to 55°C failed to show any significant improvement. The addition of p-cyanophenol to the mobile phase to suppress secondary interactions with the stationary phase did not result in the expected improvement in efficiency. (C) 2000 Elsevier Science B.V.
History
Publication title
Journal of Chromatogrophy AVolume
892Issue
1-2Pagination
303-313ISSN
0021-9673Department/School
School of Natural SciencesPublisher
ElsevierPlace of publication
AmsterdamRepository Status
- Restricted