Persistence, morphology, and nutritional state of a gastropod hosted bacterial symbiosis in different levels of hydothermal vent flux

The limpet, Lepetodrilus fucensis McLean, is found in prominent stacks around hydrothermal vents on the Juan de Fuca Ridge. L. fucensis hosts a Wlamentous episymbiont on its gill lamellae that may be ingested directly by the gill epithelium. To assess the persistence of this symbiosis I used microscopy to examine the gills of L. fucensis from sites representing its geographic range and diVerent habitats. The symbiosis is present on all the specimens examined in this study, including both sexes and a range of juvenile and adult sizes. Next, I aimed to determine if patterns in bacterial abundance, host condition, and gill morphology support the hypotheses that the bacteria are chemoautotrophic and provide limpets with a food resource. To do so, I compared specimens from high and low Xux locations at multiple vents. My results support the above hypotheses: (1) gill bacteria are signiWcantly less abundant in low Xux where the concentrations of reduced chemicals (for chemoautotrophy) are negligible, (2) low Xux specimens have remarkably poor tissue condition, and (3) the lamellae of high Xux limpets have greater surface area: the blood space and bacteria-hosting epithelium are deeper and have more folds than low Xux lamellae, modiWcations that support higher symbiont abundances. I next asked if the morphology of the lamellae could change. To test this, I moved high Xux limpets away from a vent and after 1 year the lamellar depth and shape of the transplanted specimens resembled low Xux gills. Last, I was interested in whether bacterial digestion by the gill epithelium is a signiWcant feeding mechanism. As bacteria-like cells are rarely apparent in lysosomes of the gill epithelium, I predicted that lysosome number would be unrelated to bacterial abundance. My data support this prediction, suggesting that digestion of bacteria by the gill epithelium probably contributes only minimally to the limpet’s nutrition. Overall, the persistence and morphology of the L. fucensis gill symbiosis relates to the intensity of vent Xux and indicates that specimens from a variety of habitats may be necessary to characterize the morphological variability of gill-hosted symbioses in other molluscs.