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135236 - Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method.pdf (1.43 MB)

Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method

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posted on 2023-05-20, 07:31 authored by Zhuang, T, Urakawa, M, Sato, H, Sato, Y, Taguchi, T, Umino, T, Katto, S, Tanaka, K, Yoshimura, K, Takada, N, Kobayashi, H, Ito, M, Michael RoseMichael Rose, Kiku, Y, Nagasawa, Y, Kitazawa, H, Watanabe, K, Nochi, T, Hayashi, T, Aso, H
Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a+/CD11c+/MHC (major histocompatibility complex) class II+ cells. Although DCs are identified at 0.1%–0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic‐activated cell sorting, and purified the CD172a+/CD11c+ DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co‐stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co‐stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage.

History

Publication title

Animal Science Journal

Volume

89

Issue

7

Pagination

1011-1019

ISSN

1344-3941

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Wiley-Blackwell Publishing Ltd

Place of publication

Australia

Rights statement

Copyright 2018 the authors. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/

Repository Status

  • Open

Socio-economic Objectives

Dairy cattle

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