University Of Tasmania
Physico-chemical properties of late-incubation egg amniotic fluid.pdf (342.96 kB)
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Physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed supplement

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posted on 2023-05-21, 11:41 authored by Apeh OmedeApeh Omede, Bhuiyan, MM, Islam, AF, Iji, PA


This study explored the physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed (IOF) supplement.


Amniotic fluid was collected from broiler breeders (Ross 308, 51 weeks and Cobb 500, 35 weeks) on day 17 after incubation. A mixture of high-quality soy protein supplement – Hamlet Protein AviStart (HPA) was serially diluted in MilliQ water to obtain solutions ranging from 150 to 9.375 mg/mL. The mixtures were heat-treated (0, 30, 60 minutes) in a waterbath (80°C) and then centrifuged to obtain supernatants. The amniotic fluid and HPA supernatants were analysed for their physico-chemical properties.


Only viscosity and K+ were significantly (p<0.05) different in both strains. Of all essential amino acids, leucine and lysine were in the highest concentration in both strains. The osmolality, viscosity and pCO2 of the supernatants decreased (p<0.05) with decreasing HPA concentration. Heat treatment significantly (p<0.05) affected osmolality, pH, and pCO2, of the supernatants. The interactions between HPA concentration and heat treatment were significant with regards to osmolality (p<0.01), pH (p<0.01), pCO2 (p<0.05), glucose (p<0.05), lactate (p<0.01) and acid-base status (p<0.01) of HPA solutions. The Ca2+, K+, glucose, and lactate increased with increasing concentration of HPA solution. The protein content of HPA solutions decreased (p<0.05) with reduced HPA solution concentrations. The supernatant from 150 mg/mL HPA solution was richest in glutamic acid, aspartic acid, arginine and lysine. Amino acids concentrations were reduced (p<0.05) with each serial dilution but increased with longer heating.


The values obtained in the primary solution (highest concentration) are close to the profiles of high-protein ingredients. This supplement, as a solution, hence, may be suitable for use as an IOF supplement and should be tested for this potential.


Publication title

Asian - Australasian Journal of Animal Sciences










Tasmanian Institute of Agriculture (TIA)


Asian-Australasian Assoc Animal Production Societies

Place of publication

College Agriculture Life Sciences, Dept Animal Science Technology, Suwon, South Korea, 441-744

Rights statement

© 2017 by Asian-Australasian Journal of Animal Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (CC BY-NC 4.0) (

Repository Status

  • Open

Socio-economic Objectives


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