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Post-ovulatory egg viability in the snapper Pagrus auratus (Sparidae)
journal contribution
posted on 2023-05-16, 10:42 authored by Hobby, AC, Pankhurst, NWThe role of gonadal steroids in maintaining post-ovulatory egg viability was investigated in the snapper (Pagrus auratus), a daily-repeat-spawning teleost. Ovulated eggs were held in vitro in L15 medium, or in L15 medium supplemented with gonadal steroids or gonadotrophin, to investigate any direct effects of reproductive hormones on post-ovulatory egg viability. The viability of eggs retained in the oviduct decreased with increasing time after ovulation, to give fertilization of below 50% after 6 h. The viability of eggs held in L15 medium was at least as good at that of eggs held in vivo. Storage of eggs in L15 medium supplemented with the reproductive hormones testosterone, 17α-hydroxyprogesterone, 17α,20β-dihydroxy-4-pregnen-3-one and human chorionic gonadotrophin did not improve or reduce viability relative to controls, suggesting that the hormones investigated have no direct effect on post-ovulatory egg viability.
History
Publication title
Marine and Freshwater ResearchVolume
48Issue
5Pagination
385-389ISSN
1323-1650Department/School
Institute for Marine and Antarctic StudiesPublisher
CSIROPlace of publication
AustraliaRepository Status
- Restricted