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Postcolumn derivatization of amino acids using reaction flow chromatography columns with fluorescence detection: a fast new approach to selective derivatization techniques
journal contribution
posted on 2023-05-19, 16:42 authored by Pravadali-Cekic, S, A Jones, Kazarian, AA, Brett PaullBrett Paull, Soliven, A, Ritchie, H, Camenzuli, M, Leung, L, Dennis, GR, Shalliker, RAReaction flow (RF) chromatography with fluorescamine reagent and fluorescence detection (FLD) was used for the analysis of amino acids. The performance of RF chromatography was tested against several optimized conventional postcolumn derivatization (PCD) methods. RF columns achieved greater sensitivity compared to conventional PCD methods, without the need for reaction loops, which resulted in more efficient separations. The RF-PCD method also achieved limits of detection (LOD) from the low picomole to subnanomole range. The calibration data of the RF-PCD technique yielded R2 ≥ 0.99 and % relative standard deviation in peak areas ranging from 0.34% to 5%. Through reaction flow chromatography, multiplexed detection was also achieved allowing the monitoring and analysis of derivatized and nonderivatized flow streams simultaneously.
History
Publication title
Journal of Liquid Chromatography and Related TechnologiesVolume
40Pagination
83-92ISSN
1082-6076Department/School
School of Natural SciencesPublisher
Marcel Dekker IncPlace of publication
270 Madison Ave, New York, USA, Ny, 10016Rights statement
© 2017 Taylor & FrancisRepository Status
- Restricted