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Potassium activities in cell compartments of salt-grown barley leave

Version 2 2024-09-17, 02:09
Version 1 2023-05-16, 17:57
journal contribution
posted on 2024-09-17, 02:09 authored by Tracey Cuin, AJ Miller, SA Laurie, RA Leigh
Triple-barrelled microelectrodes measuring K+ activity (aK), pH and membrane potential were used to make quantitative measurements of vacuolar and cytosolic aK in epidermal and mesophyll cells of barley plants grown in nutrient solution with 0 or 200 mM added NaCl. Measurements of aK were assigned to the cytosol or vacuole based on the pH measured. In epidermal cells, the salt treatment decreased aK in the vacuole from 224 to 47 mM and in the cytosol from 68 to 15 mM. In contrast, the equivalent changes in the mesophyll were from 235 to 150 mM (vacuole) and 79 to 64 mM (cytosol). Thus mechanisms exist to ameliorate the effects of salt on aK in compartments of mesophyll cells, presumably to minimize any deleterious consequences for photosynthesis. Thermodynamic calculations showed that K+ is actively transported into the vacuole of both epidermal and mesophyll cells of salinized and non-salinized plants. Comparison of the values of aK in K+-replete, non-salinized leaf cells with those previously measured in root cells of plants grown under comparable conditions indicates that cytosolic aK is similar in cells of both organs, but vacuolar aK in leaf cells is approximately twice that in roots. This suggests differences in the regulation of vacuolar aK, but not cytosolic aK, in leaf and root cells.

History

Publication title

Journal of Experimental Botany

Volume

54

Issue

383

Pagination

657-661

ISSN

0022-0957

Department/School

Agriculture and Food Systems

Publisher

Oxford University Press

Publication status

  • Published

Place of publication

United Kingdom

Socio-economic Objectives

260301 Barley

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