University Of Tasmania

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Precise, accurate and user-independent blood collection system for dried blood spot sample preparation

journal contribution
posted on 2023-05-20, 22:46 authored by Neto, R, Gooley, A, Michael BreadmoreMichael Breadmore, Emily HilderEmily Hilder, Lapierre, F
An accurate and precise 3 μL blood collection and dispensing system is presented for the preparation of dried blood spot (DBS) samples. Using end-to-end glass capillaries in conjugation with pre-punched DBS pads, a blood micro collection system was developed to eliminate the haematocrit dispersion, widely associated with DBS technology, while providing better levels of accuracy and precision during sample preparation. This methodology is compared to traditional micro-volume blood collection systems, such as a pipette and a digitally controlled analytical syringe. Results showed that % of recovery for the capillary methodology was closer to 100% across the three haematocrit (HCT) levels tested and when prepared by two users (98 to 100% for capillaries, 78 to 104% for pipette and 93 to 97% for digital syringe) attesting a higher accuracy. Additionally, by taking advantage of the capillary action mechanism to collect and dispense autonomously the desired volume of blood onto the DBS pad, coefficients of variation between two individuals were significantly lower than with standard methodologies (capillaries - 0.05 to 0.77%, pipette - 12.71 to 18.53% and digital syringe - 0.72 to 1.77%). This alternate aspiration and dispensing methodology could be used by different users without compromising accuracy or precision when handling low volumes of blood during the pre-analytical steps.


Australian Research Council

Trajan Scientific Australia Pty Ltd

University of South Australia


Publication title

Analytical and Bioanalytical Chemistry










School of Natural Sciences


Springer-Verlag Heidelberg

Place of publication

Tiergartenstrasse 17, Heidelberg, Germany, D-69121

Rights statement

Copyright 2018 Springer-Verlag GmbH Germany, part of Springer Nature

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  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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