University Of Tasmania

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Preliminary success using hydrogen peroxide to treat Atlantic salmon Salmo salar L. affected with experimentally induced amoebic gill disease (AGD)

journal contribution
posted on 2023-05-17, 12:28 authored by Mark AdamsMark Adams, Crosbie, PBB, Barbara NowakBarbara Nowak
Currently the only effective and commercially used treatment for amoebic gill disease (AGD) in farmed Tasmanian Atlantic salmon is freshwater bathing. Hydrogen peroxide (H2O2), commonly used throughout the aquaculture industry for a range of topical skin and gill infections, was trialled in vitro and in vivo to ascertain its potential as an alternative treatment against AGD. Under in vitro conditions, trophozoites of Neoparamoeba perurans were exposed to three concentrations of H2O2 in seawater (500, 1000 & 1500 mg.l-1) over four durations (10, 20, 30 & 60 min) each at two temperatures (12 & 18°C). Trophozoite viability was assessed immediately post exposure and after 24 hours. A concentration/duration combination of 1000 mg.l-1 for > 10 min demonstrated potent amoebicidal activity. Subsequently, Atlantic salmon mildly affected with experimentally induced AGD were treated with H2O2 at 12 and 18°C for 15 minutes at 1250 mg.l-1 and their re-infection rate compared to freshwater treated fish over 21 days. Significant differences in the percentage of filaments affected with hyperplastic lesions (in association with amoebae) and plasma osmolality were noted between treatment groups immediately post-bath. However, the results were largely equivocal in terms of disease resolution over a three week period following treatment. These data suggest that H2O2 treatment in seawater successfully ameliorated a clinically light case of AGD under laboratory conditions.


Publication title

Journal of Fish Diseases






839 -848




Institute for Marine and Antarctic Studies


Blackwell Publishing Ltd

Place of publication

9600 Garsington Rd, Oxford, England, Oxon, Ox4 2Dg

Rights statement

Copyright 2012 Blackwell Publishing Ltd

Repository Status

  • Restricted

Socio-economic Objectives

Aquaculture crustaceans (excl. rock lobster and prawns)

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