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Prenatal nicotine exposure increases hyperventilation in α4-knock-out mice during mild asphyxia
journal contributionposted on 2023-05-19, 06:29 authored by Avraam, J, Choen, G, Crago, J, Peter FrappellPeter Frappell
Prenatal nicotine exposure alters breathing and ventilatory responses to stress through stimulation of nicotine acetylcholine receptors (nAChRs). We tested the hypothesis that α4-containing nAChRs are involved in mediating the effects of prenatal nicotine exposure on ventilatory and metabolic responses to intermittent mild asphyxia (MA). Using open-flow plethysmography, we measured ventilation (V˙E) and rate of O2 consumption (V˙O2) of wild-type (WT) and α4-knock-out (KO) mice, at postnatal (P) days 1-2 and 7-8, with and without prenatal nicotine exposure (6mgkg-1day-1 beginning on embryonic day 14). Mice were exposed to seven 2min cycles of mild asphyxia (10% O2 and 5% CO2), each interspersed with 2min of air. Compared to WT, α4 KO mice had increased air V˙E and V˙O2 at P7-8, but not P1-2. Irrespective of age, genotype had no effect on the hyperventilatory response (increase in V˙E/V˙O2) to MA. At P1-2, nicotine suppressed air V˙E and V˙O2 in both genotypes but did not affect the hyperventilatory response to MA. At P7-8 nicotine suppressed air V˙E and V˙O2 of only α4 KO's but also significantly enhanced V˙E during MA (nearly double that of WT; p<0.001). This study has revealed complex effects of α4 nAChR deficiency and prenatal nicotine exposure on ventilatory and metabolic interactions and responses to stress.
Publication titleRespiration Physiology and Neurobiology
Department/SchoolInstitute for Marine and Antarctic Studies
PublisherElsevier Science Bv
Place of publicationPo Box 211, Amsterdam, Netherlands, 1000 Ae