149848 - Primordial germ cell development in the poeciliid.pdf (3.99 MB)
Download filePrimordial germ cell development in the poeciliid, Gambusia holbrooki, reveals shared features between lecithotrophs and matrotrophs
journal contribution
posted on 2023-05-21, 07:18 authored by Komeil RazmiKomeil Razmi, Jawahar PATILJawahar PATILMetazoans exhibit two modes of primordial germ cell (PGC) specification that are interspersed across taxa. However, the evolutionary link between the two modes and the reproductive strategies of lecithotrophy and matrotrophy is poorly understood. As a first step to understand this, the spatio-temporal expression of teleostean germ plasm markers was investigated in Gambusia holbrooki, a poecilid with shared lecitho- and matrotrophy. A group of germ plasm components was detected in the ovum suggesting maternal inheritance mode of PGC specification. However, the strictly zygotic activation of dnd-β and nanos1 occurred relatively early, reminiscent of models with induction mode (e.g., mice). The PGC clustering, migration and colonisation patterns of G. holbrooki resembled those of zebrafish, medaka and mice at blastula, gastrula and somitogenesis, respectively—recapitulating features of advancing evolutionary nodes with progressive developmental stages. Moreover, the expression domains of PGC markers in G. holbrooki were either specific to teleost (vasa expression in developing PGCs), murine models (dnd spliced variants) or shared between the two taxa (germline and somatic expression of piwi and nanos1). Collectively, the results suggest that the reproductive developmental adaptations may reflect a transition from lecithotrophy to matrotrophy.
Funding
Australian Research Council
Inland Fisheries Service
History
Publication title
Frontiers in Cell and Developmental BiologyVolume
10Article number
793498Number
793498Pagination
1-18ISSN
2296-634XDepartment/School
Institute for Marine and Antarctic StudiesPublisher
Frontiers Research FoundationPlace of publication
SwitzerlandRights statement
Copyright 2022 Razmi and Patil Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/Repository Status
- Open