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Production of exopolysaccharides by Antarctic marine bacterial isolates

journal contribution
posted on 2023-05-16, 14:02 authored by Mancuso Nichols, CA, Garon, S, John BowmanJohn Bowman, Raguenes, G, Guezennec, J
Aims: This study was undertaken to examine and characterize Antarctic marine bacterial isolates and the exopolysaccharides (EPS) they produce in laboratory culture. Methods and Results: Two EPS-producing bacterial strains CAM025 and CAM036 were isolated from particulate material sampled from seawater and sea ice in the southern ocean. Analyses of 16S rDNA sequences placed these isolates in the genus Pseudoalteromonas. In batch culture, both strains produced EPS. The yield of EPS produced by CAM025 was 30-fold higher at -2 and 10°C than at 20°C. Crude chemical analyses showed that these EPS were composed primarily of neutral sugars and uronic acids with sulphates. Gas chromatographic analysis of monosaccharides confirmed these gross compositional findings and molar ratios of monosaccharides revealed differences between the two EPS. Conclusions: The EPS produced by Antarctic bacterial isolates examined in this study appeared to be polyanionic and, therefore, 'sticky' with respect to cations such as trace metals. Significance and Impact of the Study: As the availability of iron as a trace metal is of critical importance in the southern ocean where it is know to limit primary production, the role of these bacterial EPS in the Antarctic marine environment has important ecological implications.

History

Publication title

Journal of Applied Microbiology

Volume

96

Issue

5

Pagination

1057-1066

ISSN

1364-5072

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Blackwell Publishing Ltd

Place of publication

Oxford, England

Repository Status

  • Restricted

Socio-economic Objectives

Oceanic processes (excl. in the Antarctic and Southern Ocean)

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