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Production of polymer monolithic capillary columns with integrated gold nano-particle modified segments for on-capillary extraction

journal contribution
posted on 2023-05-17, 17:56 authored by Currivan, S, Connolly, D, Brett PaullBrett Paull
A number of polymer butyl methacrylate-co-ethylene dimethacrylate and lauryl methacrylate-co-ethylene glycol dimethacrylate monolithic phases within capillary columns were produced with integrated gold nano-particle (GNP) modified segments for on-capillary extraction and subsequent separation of biomolecules. Photo-masking and photo-grafting techniques were used to produce an aminated zone at the start of the polymer monolithic columns, which was then converted to a nano-agglomerated section using GNPs, followed by the otherwise unmodified monolith. The extent of amination was verified using scanning capacitively coupled contactless conductivity (sC4D) and the C4D profiles of modified monoliths monitored throughout the entire fabrication process, as a quality control technique. Two approaches to monolith amination were compared for achieving maximum GNP coverage, using surface photo-grafting of either vinyl azlactone or glycidyl methacrylate, prior to reaction with ethylenediamine. The modified monolithic columns were applied to the on-column trapping and subsequent reversed-phase separation of protein standards.

History

Publication title

Microchemical Journal

Volume

111

Pagination

32-39

ISSN

0026-265X

Department/School

School of Natural Sciences

Publisher

Elsevier Inc.

Place of publication

United States

Rights statement

Copyright 2012 Elsevier B.V.

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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