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Proteomic Analysis of Selected Prognostic Factors of Breast Cancer
journal contribution
posted on 2023-05-16, 15:57 authored by Roberts, K, Bhatia, K, Peter StantonPeter Stanton, Lord, RJThe incidence of breast cancer is on the rise but as yet there is no guaranteed beneficial treatment for many of the sufferers. The treatments specific for breast and other hormone-sensitive cancers work well at times, however, the population of women that they will benefit is relatively small. Many are limited to surgical, chemotherapy, and radiotherapy options. Here, using two-dimensional electrophoresis (2-DE) in conjunction with a silver stain and Western blotting approach, we attempt to locate selected known prognostic markers for breast cancer. With these results, we can exclude these proteins from the future search for potential pharmaceutical targets, using the same techniques. The proteins that were located include the estrogen receptor-α, β-casein, cytokeratin 7, calponin and bax. For each protein an estimated M r and p/was gained. Each protein was found in multiple variants. By locating these proteins the number of unknown proteins found on the 2-DE gel has been reduced, helping the future search for novel markers that are shown as being differentially expressed between healthy and cancerous tissue samples.
History
Publication title
ProteomicsVolume
4Pagination
784-792ISSN
1615-9853Department/School
Tasmanian School of MedicinePublisher
Wiley - V C H Verlag GmbH & Co KGaAPlace of publication
GermanyRepository Status
- Restricted