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Pseudomonas siderophores in the sputum of patients with cystic fibrosis

journal contribution
posted on 2023-05-17, 11:49 authored by Martin, LW, Reid, DW, Sharples, KJ, Lamont, IL
The lungs of patients with cystic fibrosis become chronically infected with the bacterium Pseudomonas aeruginosa, which heralds progressive lung damage and a decline in health. Iron is a crucial micronutrient for bacteria and its acquisition is a key factor in infection. P. aeruginosa can acquire this element by secreting pyoverdine and pyochelin, ironchelating compounds (siderophores) that scavenge iron and deliver it to the bacteria. Siderophoremediated iron uptake is generally considered a key factor in the ability of P. aeruginosa to cause infection. We have investigated the amounts of pyoverdine in 148 sputum samples from 36 cystic fibrosis patients (30 infected with P. aeruginosa and 6 as negative controls). Pyoverdine was present in 93 samples in concentrations between 0.30 and 51 lM (median 4.6 lM) and there was a strong association between the amount of pyoverdine and the number of P. aeruginosa present. However, pyoverdine was not present, or below the limits of detection (*0.3 lM), in 21 sputum samples that contained P. aeruginosa. Pyochelin was also absent, or below the limits of detection (*1 lM), in samples from P. aeruginosainfected patients with little or no detectable pyoverdine. Our data show that pyoverdine is an important iron-scavenging molecule for P. aeruginosa in many cystic fibrosis patients, but other P. aeruginosa iron uptake systems must be active in some patients to satisfy the bacterial need for iron.

History

Publication title

Biometals

Volume

24

Issue

6

Pagination

1059-1067

ISSN

0966-0844

Department/School

Menzies Institute for Medical Research

Publisher

Kluwer Academic Publ

Place of publication

Van Godewijckstraat 30, Dordrecht, Netherlands, 3311 Gz

Rights statement

Copyright 2011 Springer Science+Business Media, LLC.

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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