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Rapid and sensitive analysis of vincristine in human plasma using on-line extraction combined with liquid chromatography/tandem mass spectrometry

journal contribution
posted on 2023-05-18, 02:48 authored by Corona, G, Casetta, B, Sandron, S, Vaccher, E, Toffoli, G
A simple and rapid method has been developed and validated for the quantitation of vincristine in human plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS) with atmospheric pressure chemical ionization using on-line solid-phase extraction. The method uses vinblastine as internal standard and the sample preparation is limited just to a plasma protein precipitation step. Further sample clean-up is carried out on-line through a perfusion column preceding an analytical phenyl LC column, the latter directly connected to the mass spectrometer. Quantitation is performed in multiple reaction monitoring mode using the transitions of m/z 825.3  → 765.3 and 811.3 → 751.3 for vincristine and vinblastine respectively. The assay was linear (r2 ≥0.99) in a concentration range from 0.1 to 500 ng/mL. Carry-over, measured on the experimental set-up, was less than 0.04%. Recovery for vincristine and the internal standard was within 90–95%. The intra-day and inter-day assay precision ranged from 1.2% to 6.8% RSD while mean percentage deviation from nominal value ranged from 0.01% to 6.1%. The proposed assay was found suitable for pharmacokinetics investigations and clinical therapeutic drug monitoring especially in pediatric cancer patients.


Publication title

Rapid Communications in Mass Spectrometry










School of Natural Sciences


John Wiley & Sons Ltd

Place of publication

The Atrium, Southern Gate, Chichester, England, W Sussex, Po19 8Sq

Rights statement

Copyright 2008 John Wiley & Sons, Ltd.

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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