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Rapid ion chromatography of L-ascorbic acid, nitrite, sulfite, oxalate, iodide and thiosulfate by isocratic elution utilizing a postcolumn reaction with cerium(IV) and fluorescence detection

journal contribution
posted on 2023-05-16, 13:52 authored by Miura, Y, Hatakeyama, M, Hosino, T, Paul HaddadPaul Haddad
Rapid separation and determination of mixtures of Image -ascorbic acid, nitrite, sulfite, oxalate, iodide and thiosulfate by conventional ion chromatography is often difficult due to incomplete separation of Image -ascorbic acid and nitrite from the water peak when using eluents giving short elution times for iodide and thiosulfate. Separation of the six species within about 15 min has been achieved by isocratic elution using a resin-based ion-exchange column with a carbonate eluent containing a trace amount of 1,3,5-benzenetricarboxylic acid (BTA) and fluorescence measurement of cerium(III) formed via postcolumn reactions of the separated sample species with cerium(IV). Calibration plots of peak height versus concentration were linear up to 10.0 μM (1.76 ppm) for Image -ascorbic acid, 8.0 μM (0.37 ppm) for nitrite, 8.0 μM (0.70 ppm) for oxalate, 80.0 μM (10.2 ppm) for iodide and 25.0 μM (2.80 ppm) for thiosulfate, whilst the sulfite calibration was linear up to 25.0 μM (2.00 ppm) when peak area was plotted against concentration. Detection limits (defined as S/N=3) were 18 ppb for Image -ascorbic acid, 4 ppb for nitrite, 16 ppb for sulfite, 7 ppb for oxalate, 72 ppb for iodide and 37 ppb for thiosulfate. The proposed method was applied successfully to the determination of Image -ascorbic acid, nitrite, sulfite, oxalate, iodide or thiosulfate in water samples.

History

Publication title

Journal of Chromatography A

Volume

956

Issue

1-2

Pagination

77-84

ISSN

0021-9673

Department/School

School of Natural Sciences

Publisher

Elsevier Science BV

Place of publication

Amsterdam, The Netherlands

Rights statement

The definitive version is available at http://www.sciencedirect.com

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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