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Recirculating and marginal zone B cell populations can be established and maintained independently of primary and secondary follicles
journal contributionposted on 2023-05-17, 07:45 authored by Heinrich KornerHeinrich Korner, Winkler, TH, Sedgwick, JD, Rollinghoff, M, Basten, A, Cook, MC
In normal spleen, most recirculating naÃ¯ve IgM +IgD hi B cells are located within primary follicles and mantle zones of secondary follicles. By contrast, the marginal zone contains a heterogeneous population of IgM hi- IgD lo/- B cells that are mostly non-recirculating. Although these are dynamic populations they are maintained at a constant size, the fundamental homeostatic mechanisms remain uncertain. One possibility is that the presence and turnover of each of the B cell populations is dependent on their location within discrete splenic compartments. To investigate this, we have characterized immature, non-recirculating, mature recirculating, marginal zone and B-1 cell populations in TNF -/- and TNF/lymphotoxin(LT)-Î± -/- mice that have disorganized splenic architecture. Labelling with 5-bromo-2â€²-deoxyuridine revealed that turnover of B cells in TNF -/- mice is normal, but is diminished in TNF/LT-Î± -/- mice. The recirculating B cell populations in both mutant strains are normal in proportion and phenotype. Marginal zone B cells are not seen in TNF/LT-Î± -/- mice, but this population appears normal in TNF -/- mice, even though they lack germinal centres. These findings indicate that peripheral B cell subsets can be established and maintained independently of normal follicular architecture.
Publication titleImmunology and Cell Biology
Department/SchoolMenzies Institute for Medical Research
PublisherBlackwell Publishing Asia
Place of publication54 University St, P O Box 378, Carlton, Australia, Victoria, 3053