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Redistribution of carbonic anhydrase VI expression from ducts to acini during development of ovine parotid and submandibular glands
journal contribution
posted on 2023-05-16, 10:49 authored by Penschow, JD, Giles, ME, Coghlan, JP, Fernley, RTCarbonic anhydrase VI (CA VI) is a secreted enzyme produced predominantly by serous acinar cells of submandibular and parotid glands. We have investigated the developmental pattern of CA VI production by these glands in the sheep, from fetal life to adulthood, using immunohistochemistry. Also, a specific radioimmunoassay for CA VI was used to measure changes in enzyme expression in the parotid gland postnatally. CA VI is detectable by immunohistochemistry in parotid excretory ducts from 106 days gestation (term is 145 days), in striated ducts from 138 days and in acinar cells from I day postnatal. The duct cell content of CA VI declined as the acinar cell population increased, a feature also of CA VI immunoreactivity in the submandibular gland. Production of CA VI by submandibular duct cells was detectable initially at 125 days gestation, and acinar production was not seen before 29 days post-natal. Apart from the differing ontogeny of CA VI production in ducts and acini of parotid and submandibular glands, there was a parallel pattern of CA VI expression during the development of these major salivary glands. With the development of the acinar tissues in the postnatal lamb, there was a dramatic increase (about 600-fold) in the level of expression of CA VI in the parotid gland between days 7 and 59 as measured by radioimmunoassay.
History
Publication title
Histochemistry and Cell BiologyVolume
107Issue
5Pagination
417-422ISSN
0301-5564Department/School
Tasmanian School of MedicinePublisher
Springer VerlagPlace of publication
UKRepository Status
- Restricted