Release of extracellular purines from plant roots and effect on ion fluxes
journal contribution
posted on 2023-05-17, 07:32authored byDark, A, Demidchik, V, Richards, SL, Sergey ShabalaSergey Shabala, Davies, JM
Extracellular purine nucleotides appear capable of regulating plant development, defense and stress responses by acting in part as agonists of plasma membrane calcium channels. Factors stimulating ATP release include wounding, osmotic stress and elicitors. Here we show that exogenous abscisic acid and L-glutamate can also cause ATP accumulation around Arabidopsis thaliana roots. Release of ADP from root epidermis would trigger ionotropic receptor-like activity in the plasma membrane, resulting in transient elevation of cytosolic free calcium. Root epidermal protoplasts (expressing aequorin as a cytosolic free calcium reporter) can support an extracellular ADP-induced cytosolic calcium elevation in the presence of an extracellular reductant. This confirms that ADP could elicit calcium-based responses distinct to those of ATP, which have been shown previously to involve production of extracellular reactive oxygen species.
History
Publication title
Plant Signalling & Behavior
Volume
6
Issue
11
Pagination
1855-1857
ISSN
1559-2316
Department/School
Tasmanian Institute of Agriculture (TIA)
Publisher
Landes Bioscience
Place of publication
United States
Rights statement
Copyright 2011 Landes Biosciences
Repository Status
Restricted
Socio-economic Objectives
Other plant production and plant primary products not elsewhere classified