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Retention behavior and selectivity of a latex nanoparticle pseudostationary phase for electrokinetic chromatography
journal contribution
posted on 2023-05-17, 07:29 authored by Palmer, CP, Keeffer, A, Emily HilderEmily Hilder, Paul HaddadPaul HaddadThe retention characteristics and separation selectivity of a novel latex nanoparticle (NP) pseudostationary phase (PSP) for electrokinetic chromatography have been characterized. The anionic NPs have very low or no affinity for cationic solutes, but show significant interactions and retention based on hydrophobic interactions. Retention factors of alkyl-phenyl ketones increase linearly with the concentration of the NPs and have zero or near zero y-intercepts as expected for electrokinetic chromatography with non-micellar PSPs. The retention factors of these solutes and representative pharmaceuticals decrease logarithmically with increases in the concentration of ACN in the background electrolyte, as expected for reversed-phase retention. Linear solvation energy relationship analysis indicates that the NPs are less cohesive than would be expected for polymeric PSPs with similar structure but that the overall separation selectivity can be expected to be similar to polymer PSPs with similar backbone chemistry. The results indicate that the hydrophobic core of the NPs is non-cohesive and is highly accessible to solutes, whereas the ionic head groups are not as accessible and do not contribute substantially to retention or selectivity.
Funding
Australian Research Council
History
Publication title
ElectrophoresisVolume
32Issue
5Pagination
588-594ISSN
0173-0835Department/School
School of Natural SciencesPublisher
Wiley-V C H Verlag GmbhPlace of publication
Weinheim, GermanyRights statement
The definitive published version is available online at: http://www3.interscience.wiley.com/Repository Status
- Restricted