Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: A PCR validation

<p><strong>Aim: </strong>In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (<i>NQO1</i>); γ-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (<i>Nox</i>)-4; <i>Nox2B</i>) genes. </p> <p><strong>Results: </strong>Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely <i>Nox2B</i> and <i>Nox4</i>, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and <i>Nqo-1</i> in a dose-dependent manner. </p> <p><strong>Conclusion: </strong>The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.</p>