Expression of TCF/LEF-family genes in E11
Taken from "Hyperactive Wnt signaling changes the developmental potential of embryonic lung endoderm"
Journal of Biology 2004;3(3):11-11.
Published online 8 Jun 2004
Copyright © 2004 Okubo and Hogan, licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.5 lung endoderm. RT-PCR analysis of TCF/LEF family genes in distal and proximal endoderm. E11.5 lungs were collected, dissected into trachea and primary bronchi (proximal region), and the remainder (distal region) and endoderm was separated from mesoderm using enzymatic treatment. Total RNA isolated from whole lungs (W) and proximal (P) and distal (D) endoderm was used for RT-PCR. The absence of RNA from the endoderm fractions confirms the removal of the mesoderm. Lef1 protein is localized in the nuclei of lung epithelial cells at E14.5 (b), and nuclei are also stained with DAPI (c). The images are merged in (d); the bar is 50 μm.