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Single-cell profiling identifies key pathways expressed by iPSCs cultured in different commercial media

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posted on 2023-05-19, 23:56 authored by Daniszewski, M, Nguyen, Q, Chy, HS, Vikrant Singh, Crombie, DE, Kulkarni, T, Liang, HH, Sivakumaran, P, Lidgerwood, GE, Hernandez, D, Conquest, A, Rooney, LA, Chevalier, S, Andersen, SB, Senabouth, A, James VickersJames Vickers, Mackey, DA, Craig, JE, Laslett, AL, Alexander HewittAlexander Hewitt, Powell, JE, Pebay, A
We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells from four samples consisting of two different iPSC lines each grown in the two culture media revealed a shared subpopulation structure with three main subpopulations different in pluripotency states. By implementing a machine learning approach, we estimated that most cells within each subpopulation are very similar between all four samples. The single-cell RNA sequencing analysis of iPSC lines grown in both media reports the molecular signature in StemFlex medium and how it compares to that observed in the TeSR-E8 medium.

History

Publication title

iScience

Volume

7

Pagination

30-39

ISSN

2589-0042

Department/School

Menzies Institute for Medical Research

Publisher

Cell Press

Place of publication

United States

Rights statement

Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

Repository Status

  • Open

Socio-economic Objectives

Clinical health not elsewhere classified

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