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Spatial Distribution of Nutritive and Nonnutritive Vascular Routes in Perfused Rat Hindlimb Muscle Using Microspheres
journal contributionposted on 2023-05-16, 12:53 authored by Michelle Keske, Stephen RattiganStephen Rattigan, Michael ClarkMichael Clark, Bernard, SL, Glenny, RW
Skeletal muscle appears to have two vascular flow routes, nutritive and nonnutritive, where the balance of flow is controlled by vasoconstrictors. In the present study, spatial distributions of the two flow routes in muscles of the perfused rat hindlimb were attempted using fluorescent microspheres (15 Î¼m in diameter). Microspheres were injected during steady-state perfusion with norepinephrine (proposed recruiter of nutritive flow), serotonin (proposed recruiter of nonnutritive flow), or vehicle. The three-dimensional location of individual microspheres in representative muscles was determined using a Fluorescent Imaging CryoMicrotome. Norepinephrine and serotonin each increased perfusion pressure (P < 0.05) but stimulated and inhibited oxygen consumption (P < 0.05), respectively. The distribution of microspheres lodged in muscle was independent of the agent used. Spatial perfusion indices for norepinephrine, serotonin, and vehicle did not differ from each other. Similarly, there was no difference in these indices for a theoretical distribution where microspheres were deliberately positioned in muscle bundle capillaries or interfibrillar connective tissue vessels. We conclude that the nutritive and nonnutritive flow routes are distributed throughout muscle sections consistent with their locations in muscle bundle capillaries and interfibrillar connective tissue, respectively. Â© 2000 Academic Press.
Publication titleMicrovascular Research
Department/SchoolTasmanian School of Medicine
PublisherAcademic Press Inc.
Place of publicationSan Diego, USA